In order to analyze the Os1bglu4 phenotype, the inducible promoter of the transgenic rice which knock-down the Os1bglu4 expression was assessed. The re-sult showed that 30 μM dexamethasone(DEX) had the stronger induction effect than 10 μM DEX by β-Glucuronidase (GUS) staining. qRT-PCR further verified the Os1bglu4 gene deletion. The effect of DEX and its solvent absolute ethanol on seed development was measured, and no significant effect was observed. The con-clusion is that final concentration of DEX at 30 μM is suitable for pOp6 promoter in-duction.