Nutrient addition has been proved to be an effective strategy to enhance oil biodegradation in marine shorelines. To determine the optimal range of nutrient concentrations in the bioremediation of oil-polluted beaches, nitrate was added to the simulated shoreline models in the initial concentration of 1, 5 and 10 mg/L. Whenever the NO3-N concentration declined to 70% of its original value, additional nutrients were supplemented to maintain a certain range. Results showed adding nutrients increased the oil biodegradation level, the counts of petroleum degrading bacteria (PDB) and heterotrophic bacteria (HB), and the promoted efficiency varied depending on the concentration of nitrate. Oil degradation level in 5 mg/L (NO3-N) group reached as much as 84.3% accompanied with the consistently highest counts of PDB; while in 1 mg/L group oil removal efficiency was only 35.2%, and the numbers of PDB and HB were relatively low compared to the other groups supplemented with nutrients. Although counts of HB in the 10 mg/L group were remarkable, lower counts of PDB resulted in poorer oil removal efficiency (70.5%) compared to 5 mg/L group. Furthermore, it would need more NO3-N (0.371 mg) to degrade 1 mg diesel oil in the 10 mg/L group than in the 5 mg/L group (0.197 mg). In conclusion, Nitrate concentration in 5 mg/L is superior to 1 and 10 mg/L in the enhancement of diesel oil biodegradation in simulated shorelines.