The dynamic temperature variation of bio-materials induced by pulsed-laser irradiation is determined by a rapid transient temperature measuring system. The heat transfer characteristics of bio-materials, and the influence of pulse duration, power density, thickness and initial moisture content of bio-materials on heat transfer are studied. Based on the experiments, a mathematical model of bioheat transfer is set up. Numerical simulation results show that the calculated results agree well with the experimental results, and bioheat transfer characteristics irradiated by pulsed-laser can be displayed well. Valuable information and experiences are provided for further experimental and theoretical investigation.