The reflecting events from Moho and other interfaces within the crust are recognized from the wavefield characteristics of P- and S-wave for the 480km long wide-angle seismic profile between Peigu Tso and Pumoyong Tso. Then, seismic crustal structures of P- and S-wave velocities and Poisson ratio under the nearly east-west profile in southern Tibet are interpreted by fitting the observed traveltimes with the calculated ones by forward modelling. Our interpreting results demonstrate that the crustal thickness varies remarkably in the east-west direction, showing a pattern that the crust could be divided into three parts bounded by the west of Dingri and the east of Dinggyê, respectively, where the depth of Moho is about 71km for the western part, about 76km for the middle and about 74km for the eastern. There is one lower velocity layer (LVL) at the bottom of the upper crust with depth of 20-30 km. One of the distinct features is that the thickness of LVL abruptly thins from 24km on the west to 6km on the east. The other is that the velocity variation in the crust along east-west direction for both P- and S-wave displays a feature as quasi-periodic variation. The lower velocity (compared to the average value for the continent of the globe) in the lower crust and three sets of north-southward active normal faults are probably attributed to the coupling process of material delamination in the lower crust, crustal thicking and east-westward escape of the crustal material accompanied with the continental collision between India and Eurasia Plate.