The effects of natural fish oil, DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream (Sparus aurata) were investigated after 15 days feeding trials. The tolerance of larval gilthead seabream to various stress factors such as exposure to air ( lack of dissolved oxygen), changes in water temperature (low) and salinity ( high) were determined. This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for increasing the tolerance of larval gilthead seabream to various stresses, and that microparticulate diet with natural fish oil and palmitic acid (16:0) was more effective than microparticulate diet with DHA oil and soybean lecithin.